A dead fox was brought in to the Scottish Natural Heritage (SNH) office in Lerwick on Tuesday morning after having been found by a member of the public at Shurton Brae, Gulberwick.
The animal, believed to be a young male, is suspected to have been hit by a vehicle on the road, although SNH officers are currently awaiting results from a post mortem.
Glen Tyler from SNH said there is no known wild population of foxes in Shetland. One was introduced to the isle around 100 years ago, but since 1995 there have been only three sightings of foxes in the isle, although these have all been dead.
The last one to be found was in 2008, at the side of the road near Scatsta airport.